Methods Suspension of PMSC which obtained from cell culture of periosteal segments in vitro were injected into the backs of nude mice subcutaneously, and the fracture site of neck of femur in old person. 方法将幼儿骨膜分离培养后获得的骨膜间质干细胞悬液,移植于裸小鼠两侧背部皮下及老年股骨颈骨折处,观察并验证新骨形成。